Carnitine Acetyltransferase from pigeon breast muscle 

Protein determined by biuret

Carnitine acetyltransferase maintains the cellular and mitochondrial levels of acetyl-CoA, a key cofactor required for oxidative metabolism, by catalyzing an equilibrium between acetyl-CoA and acetyl-L-carnitine, a storage form of activated acetate. Carnitine acetyltransferase also maintains the pool of acetyl-CoA required for neuronal and nonneuronal acetylcholine production.

One unit will convert 1.0 μmole of acetyl-L-carnitine and CoA to L-carnitine and acetyl-CoA per min at pH 8.0 at 25 °C.

Crystalline suspension in 2.9 M (NH₄)₂SO₄ with 50 mM potassium phosphate and 1 mM EDTA, pH ~7.5

ammonium sulfate suspension

2-8°C

Ramsay, R.R., and Naismith, J.H., A snapshot of carnitine acetyltransferase. Trends Biochem. Sci. 28, 343-346, (2003)

Bakker, A., et al., Ultrastructural localisation of carnitine acetyltransferase activity in mitochondria of rat myocardium. Biochim. Biophys. Acta 1185, 97-102, (1994)

Horiuchi, Y., et al., Evolutional study on acetylcholine expression. Life Sci. 72, 1745-1756, (2003)

1

E3876, N-Ethylmaleimide

I1149, Iodoacetamide

I6125, Iodoacetamide