Immunogen
cytoskeletal preparation from rat colon epithelium.
Specificity
Recognizes specifically human cytokeratin 17 (46-47 kDa) and rat cytokeratin 19 (40 kDa). The antibody labels intermediate filaments in human cultured epitheloid carcinoma cell line (HeLa) and in rat hepatoma cells, but does not stain rat fibroblasts in primary cultures. It stains a minor basal cell population in all variants of complex epithelia; strong staining was noted in myoepithelial cells of all glands studied. The antibody exhibits a wide interspecies cross-reactivity, however, the recognized cytokeratin was not studied in cases other than human and rat. The epitope detected by the antibody is sensitive to formalin fixation.