Anti-Cytochrome c antibody produced in sheep 

Cytochrome c is an electron transport protein released from mitochondria as an early committed event in apoptosis. Cytochrome c and dATP are cofactors for the mammalian apoptosome, which is composed of Apaf-1, Bcl-2, and procaspase 9. When caspase 9 is activated, activation of other caspases follow including the death protease caspase 3. The release of cytochrome c is inhibited by the presence of Bcl-2 on these organelles preventing the initiation of apoptosis. In cells induced by several apoptotic agents (such as UV irradiation, staurosporine, and overexpression of Bax), caspase inhibitors do not prevent cytochrome c release.

rabbit cytochrome c.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

affinity isolated antibody

buffered aqueous solution

antigen mol wt 15 kDa

human, rat, rabbit, canine

~0.5 mg/mL

immunoblotting: 0.1-0.2 μg/mL using whole extracts of MCF-7, Jurkat, Rat-1, and MDCK cells

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using human heart tissue

indirect immunofluorescence: 5-10 μg/mL using human MCF-7 cells

human ... CYCS(54205)

rat ... Cycs(25309)

dry ice

−20°C

3