CAPHOS
Sigma

Calcium Phosphate Transfection Kit

Descriptions

Analysis Note

Tested for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

Application

Calcium phosphate transfection is a popular method for introduction of DNA into cells in culture. The method is suitable for transient as well as stable transfection.

Principle

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl₂ solution containing the DNA. A DNA−calcium phosphate coprecipitate forms which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Properties

usage

1 kit sufficient for 300 transfections (3.5 cm dishes)

1 kit sufficient for 40 transfections (10 cm dishes)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Graham, F.L. and Van der Eb, A.J., A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456-467, (1973) abstract

Wigler, MJ., et al., Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell 14, 725-731, (1978) abstract