Caspase 6 Assay Kit, Fluorimetric 

The Caspase 6 Fluorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Val-Glu-Ile-Asp-7-amino-4-methyl coumarin (Ac-VEID-AMC) by caspase 6 that results in the release of a 7-amino-4-methyl coumarin (AMC) moiety. The excitation and emission wavelengths of AMC are 360 nm and 440 nm, respectively. The concentration of the AMC released can be calculated from a calibration curve prepared from AMC standards (included with the kit).
The kit detects caspase 6 activity in crude and purified preparations of caspase 6.
Complete kit contains:
• purified caspase 6 for a positive control
• caspase 6 inhibitor
• AMC fluorescent standard

The kit provides sufficient reagents for 100 tests of 100 μl in 96-well plates.

dry ice

−20°C

Villa P., et al. Trends Biochem. Sci. 22, 388-392, (1997)

Acetyl-Val-Glu-Ile-Asp-Aldehyde Inhibitor 0.25 mL

Acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin Substrate 0.25 mL

7-Amino-4-methylcoumarin Standard 1 mL

Caspase-6 5 μg