Caspase 8 Assay Kit, Colorimetric 

The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
• purified caspase 8 for a positive control
• caspase 8 inhibitor
• p-NA fluorescent standard

The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.

dry ice

−20°C

Villa P., et al. Trends Biochem. Sci. 22, 388-392, (1997)

Acetyl-Ile-Glu-Thr-Asp-Aldehyde Inhibitor 100 μL A6464

Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL

Caspase 8, human, 5 μg

Water 125 mL

10X Assay Buffer 5 mL

5X Lysis Buffer 5 mL