Catalase Assay Kit 

The Catalase Assay Kit is a simple colorimetric assay for studying catalase activity in various tissues and subcellular organelles.

Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H₂O₂ from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.

• Useful for determining catalase activity - may be used in various tissues and cells
• Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity

One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.

sufficient for ≥100 tests enzymatic

wet ice

2-8°C

Deisseroth, A., and Dounce, A.L., Catalase: Physical and chemical properties, mechanism of catalysis, and physiological role. Physiol. Rev. 50, 319-375, (1970) abstract

Ding, M., et al., Toxoplasma gondii catalase: are there peroxisomes in toxoplasma? J. Cell Sci. 113, 2409-2419, (2000) abstract

Bai, J., et al., Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury. J. Biol. Chem. 274, 26217-26224, (1999) abstract

Hampton, M.B., and Orrenius, S., Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis. FEBS Lett. 414, 552-556, (1997) abstract

Tome, M.E., et al., Catalase-overexpressing thymocytes are resistant to glucocorticoid-induced apoptosis and exhibit increased net tumor growth. Cancer Res. 61, 2766-2733, (2001)

Kowaltowski, A.J., et al., Catalases and thioredoxin peroxidase protect Saccharomyces cerevisiae against Ca(2+)-induced mitochondrial membrane permeabilization and cell death. FEBS Lett. 473, 177-182, (2000) abstract

Tada-Oikawa, S., et al., Generation of hydrogen peroxide precedes loss of mitochondrial membrane potential during DNA alkylation-induced apoptosis. FEBS Lett. 442, 65-69, (1999) abstract

Zhou, Z., and Kang, Y.J., Cellular and subcellular localization of catalase in the heart of transgenic mice. J. Histochem. Cytochem. 48, 585-594, (2000) abstract

Zamocky, M., and Koller, F., Understanding the structure and function of catalases: clues from molecular evolution and in vitro mutagenesis. Prog. Biophys. Molec. Biol. 72, 19-66, (1999)

Assay Buffer 10X 100 mL

Chromagen Reagent 1 vial

Stop Solution 100 mL

Catalase Positive Control .25 mL

Hydrogen peroxide solution, 3 % (w/w) 10 mL

Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, ~1000 units/mg solid (using ABTS), 250-330 units/mg solid (using pyrogallol) 5 mg P6782