cGMP Enzyme Immunoassay Kit, Direct 

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

sufficient for 96 assays

wet ice

−20°C

Chard, T. An Introduction to Radioimmunoassay and Related Techniques 4th ed., Amsterdam , (1990)

Tijssen, P. Practice and Theory of Enzyme Immunoassays Amsterdam , (1985)

UN 3316 9/PG 3

0.1 M Hydrochloride Acid 1×30 mL

Acetic Anhydride 1×1 mL

cGMP-Alkaline Phosphatase Conjugate 1×5 mL

cGMP EIA Antibody Rabbit Anti-cGMP 1×5 mL

Cyclic GMP Standard 1×0.5 mL

Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 each

Neutralizing Reagent 1×6 mL

p-Nitrophenyl Phosphate Substrate Solution 1×20 mL

Plate Sealer 1 each

Stop Solution 1×5 mL

Triethylamine 1×2 mL

Wash Buffer Concentrate 1×30 mL