PCR Core kit with Taq DNA polymerase 

This kit contains all the reagents necessary to amplify genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the Polymerase Chain Reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl₂ will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg²⁺ concentrations and the cycling parameters.

The following reagents are required but not provided in the kit:
• Mineral Oil, M 8662 (optional)
• Thermocycler
• Primers
• DNA to be amplified
• Chloroform, C 7559 (optional)

Kit contains enough reagents for 250 units of Taq DNA polymerase.

Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler.

wet ice

−20°C

Taq DNA polymerase 250 units

10X PCR Buffer 1 vial P2192

10X PCR Buffer without MgCl₂ 1 vial P2317

Magnesium chloride solution, PCR Reagent 1 vial M8787

Water, PCR Reagent 3 vials W1754

Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent 0.25 ml D7295

Detailed technical bulletin