CS0030
Sigma

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

1 of 1

Descriptions

Application

Histochemical staining of β-galactosidase activity is performed at pH 6.0. Under these conditions, β-galactosidase is a biomarker specific for senescent cells, but is not found in quiescent, immortal, or tumor cells.

Biochem/physiol Actions

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Legal Information

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Properties

usage

sufficient for 100 tests

shipped in

wet ice

storage temp.

−20°C

References

Reference

Dimiri, G.P., et al., A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 92, 9363-9367, (1995) abstract

van der Loo, B., et al., Cytochemical detection of a senescence-associated β-galactosidase in endothelial and smooth muscle cells from human and rabbit blood vessels. Exp. Cell Res. 241, 309-315, (1998) abstract

Safety

Hazard Codes

Risk Statements

20/21/22-36/37/38-40-42/43

Safety Statements

23-26-36/37-45

RIDADR

UN 3316 9/PG 3

Components

Standard component

X-gal solution 4 mL

Staining Solution, 10X 15 mL