Retinoblastoma ELISA human 

The sensitivity is ~0.1 ng/mL, 20 times more sensitive than immunoblotting.

Designed for the quantitative determination of human retinoblastoma (Rb).

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Rb ELISA is designed for the quantitative detection of Rb in human cell lysates independent of its phosphorylation state. It may be used to normalize the results of Phospho-Rb (pSer²⁴⁹/pThr²⁵²) ELISA (CS0060).

Rb, the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that plays an important role in regulating cell growth and differentiation. The cell cycle inhibitory function is abrogated when Rb undergoes phosphorylation, catalyzed by the complex of cyclins/cyclin-dependent protein kinases (cdks).

The kit offer a fast (total assay time 4 hours), sensitive (sensitivity 0.1 ng/mL for non-phosphorylated and <0.8 units/mL for phosphorylated Rb) and easy alternative to immunoblotting or other bioassays. Precoated plates are provided in the kit, incubations proceed at room temperature and no proprietary equipment is required.

sufficient for 96 multiwell tests

human ... RB1(5925)

wet ice

2-8°C

Kaelin, W.G., Functions of the retinoblastoma protein. Bioessays 21, 950-958, (1999)

UN 3316 9/PG 3

Retinoblastoma Standard, Lyophilized

Standard Diluent Buffer

Monoclonal Anti-Rb-Coated 96 well plate

Monoclonal-Anti-Rb Biotin-conjugate

Streptavidin-HRP Concentrate, 100X

HRP Diluent

Wash Buffer Concentrate, 25X

Stabilized Chromogen (TMB)

Stop Solution

Plate Covers