CS0080
Sigma

Epidermal Growth Factor Receptor ELISA human

sufficient for 96 tests

Synonym

EGFR ELISA

Epidermal Growth Factor Receptor ELISA

Descriptions

Analysis Note

Specifically measures both phosphorylated and non-phosphorylated forms of EGFR with a sensitivity of <0.08 ng/mL, which is 10 times more sensitive than immunoblotting.

Application

Epidermal Growth Factor Receptor ELISA detects total EGFR in cell lysates.

Biochem/physiol Actions

EGFR, a member of the family of receptor tyrosine kinases, is a full length, type I transmembrane glycoprotein. In vivo phosphorylation of EGFR specific tyrosine sites, including Tyr¹¹⁷³ or Tyr¹⁰⁶⁸, activates EGFR and downstream molecules.

Features and Benefits

EGFR ELISAs are fast (total time 4 hours), sensitive, and specific assays. The kit contains precoated plates, all incubations proceed at room temperature, and no proprietary equipment is required.

Properties

usage

sufficient for 96 tests

Gene Information

human ... EGFR(1956)

shipped in

wet ice

storage temp.

2-8°C

References

Reference

Wells, A., EGF receptor. Int. J. Biochem. Cell Biol. 31, 637-643, (1999)