CS0150
Sigma

phospho-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸) ELISA human

sufficient for 96 tests

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Synonym

EGFR ELISA

Epidermal Growth Factor Receptor ELISA

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Descriptions

Analysis Note

Specifically measures human EGFR protein phosphorylated on tyrosine 1068 with a sensitivity of <0.3 units/mL, which is 8 times more sensitive than immunoblotting. Does not detect non-phosphorylated EGFR.

Application

phospho-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸) ELISA detects EGFR phosphorylated at tyrosine 1068 in cell lysates and biological fluids.

Biochem/physiol Actions

EGFR, a member of the family of receptor tyrosine kinases, is a full length, type I transmembrane glycoprotein. In vivo phosphorylation of EGFR specific tyrosine sites, including Tyr¹¹⁷³ or Tyr¹⁰⁶⁸, activates EGFR and downstream molecules.

Features and Benefits

EGFR ELISAs are fast (total time 4 hours), sensitive, and specific assays. The kit contains precoated plates, all incubations proceed at room temperature, and no proprietary equipment is required.

Properties

usage

sufficient for 96 tests

Gene Information

human ... EGFR(1956)

shipped in

wet ice

storage temp.

2-8°C

References

Reference

Bishayee, A. et al., Phosphorylation of tyrosine 992, 1068, and 1086 is required for conformational change of the human epidermal growth factor receptor C-terminal tail. Mol. Biol. Cell 10, 525-536, (1999) abstract

Wells, A., EGF receptor. Int. J. Biochem. Cell Biol. 31, 637-643, (1999)

Schooler, K., and Wiley, H.S, Ratiometric assay of epidermal growth factor receptor tyrosine kinase activation. Anal. Biochem. 27, 135-142, (2000)

Safety

RIDADR

UN 3316 9/PG 3

Components

Kit component only

Phospho-EGFR (pTyr¹⁰⁶⁸) Standard, lyophilized

Standard Diluent Buffer

Monoclonal Anti-EGFR-coated 96 well plate

Anti-Phospho EGFR (pTyr¹⁰⁶⁸)

Anti-rabbit IgG-HRP