Substance P EIA 

Substance P EIA is fast (total incubation time 3 hours), specific (measures levels with no cross-reactivity with neurokinins and somatostatins), and sensitive (sensitivity less than 8.04 pg/mL). All components are stable at 2-8 ºC. Sample recovery for various matrices, ranges from 81% (cell culture supernatants diluted 1:2) to 100% (porcine heparin plasma). High assay precision, intra-assay CV 5.2-6.7% and inter-assay CV 4.2-7.3%.

Substance P is an 11 amino acid (H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2) peptide that is synthesized in ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide belongs to the tachykinin family and is widely distributed in the peripheral and central nervous systems of vertebrates, where it acts as a neurotransmitter. Substance P is involved in pain, anxiety, depression and inflammation.

Substance P, widely distributed in the peripheral and central nervous systems, acts as a neurotransmitter involved in pain, anxiety, depression, and inflammation.

sufficient for 96 multiwell tests

dry ice

−20°C

Cao, Y. K, Primary afferent tachykinins are required to experience moderate to intense pain. Nature 392, 390-393, (1998)

Liu, H., et al, Resistance to excitotoxin-induced seizures and neuronal death in mice lacking the preprotachykinin A gene. Proc. Natl. Acad. Sci. USA 96, 12096-12101, (1999)

UN 3316 9/PG 3

Substance P standard, synthetic peptide

Anti-Rabbit IgG-coated 96 well plate

Assay Buffer

Anti-Substance P

Substance P-Alkaline Phosphatase Conjugate

p-Nitrophenylphosphate (pNpp) Substrate

Wash Buffer Concentrate

Stop Solution

Plate sealer