Glutathione Assay Kit 

Live Chat and Frequently Asked Questions are available for this Product.

• A quick and simple method
• The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
• The kit includes a deproteination reagent for biological samples

Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues

The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.

sufficient for 700 assays

wet ice

2-8°C

Akerboom, T.P., and Sies, H., Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol. 77, 373-382 , (1981) abstract

Assay Buffer 5x 30 mL

DTNB 8 mg

DMSO 7.5 mL

Glutathine Reductase 20 un

L-Glutathione Reduced 300 μg

NADPH 25 mg

5-Sulfosalicylic Acid 2.5 g