Caspase 2 Fluorescein (FLICA) Assay 

FLICA Caspase Assay detects active caspases inside the cell using novel Fluorochrome Inhibitor of Caspases (FLICA)-cell permeable and non-cytotoxic caspase inhibitors. Once inside the cell, the FLICA probe covalently binds to a reactive cysteine residue on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. Bound FLICA is retained within the cell, while any unbound reagent is washed away. The bound FLICA emits a green fluorescent signal, which is a direct measurement of the number of active caspase molecules that are present in the cell at the time the reagent was added.

Once reconstituted, the unused 150X FLICA stock should be stored in aliquots at −20 °C protected from light.

The FLICA Assays offer:
• Potent, cell-permeable and non-toxic fluorochrome inhibitor
• Preferential detection of selected caspases based on inhibitor specificity
• A direct measure of apoptosis expressed as the number of active caspase enzymes present in the cell

Fluorometric detection of active cellular caspase 2 using cell-permeable, non-toxic FAM-VDVAD-FMK fluorochrome inhibitor of caspase 2 that covalently binds to the enzyme and is visualized by fluorometry, fluorescence microscopy, or flow cytometry.

sufficient for 100 tests

2-8°C

Lassus, P., et al., Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 297, 1352-1354, (2002) abstract

Ekert, P.G., et al., Caspase inhibitors. Cell Death Differ. 6, 1081-1086, (1999)

Kumar, S., Mechanisms mediating caspase activation in cell death. Cell Death Differ. 6, 1060-1066, (1999)

FAM-VDVAD-FMK FLICA reagent 4 vials

Wash Buffer, Concentrate 10X 60 mL

Fixative 6 mL

Propidium iodide 1 mL

Hoechst Stain 33342 1 mL