Glutathione-S-Transferase (GST) Assay Kit 

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

The assay is intended for the measurement of the total GST activity in cell and bacterial lysates, tissue homogenates, and plasma and erythrocytes lysates.

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

sufficient for 500 multiwell tests

human ... GSTM1(2944)

dry ice

−20°C

Habig, W.H., et al., Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139, (1974) abstract

UN 3441 6.1/PG 2

Dulbecco's Phosphate Buffered Saline 100 mL

L-Glutathione Reduced 1 g

GST (Control) 0.1 mL

Sample Buffer 5 mL

Substrate (CDNB) 1.2 mL