phospho-Retinoblastoma (pThr⁸²¹) ELISA human 

The sensitivity is ~1 unit/mL of Rb (pThr⁸²¹), which is 25 times more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

Designed for the quantitative determination of human retinoblastoma (Rb).

phospho-Rb (pThr⁸²¹) ELISA detects human retinoblastoma phosphorylated on threonine 821 in cell lysates.

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Rb, the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that plays an important role in regulating cell growth and differentiation. The cell cycle inhibitory function is abrogated when Rb undergoes phosphorylation, catalyzed by the complex of cyclins/cyclin-dependent protein kinases (cdks).

The kit offer a fast (total assay time 4 hours), sensitive (sensitivity 0.1 ng/mL for non-phosphorylated and <0.8 units/mL for phosphorylated Rb) and easy alternative to immunoblotting or other bioassays. Precoated plates are provided in the kit, incubations proceed at room temperature and no proprietary equipment is required.

sufficient for 96 determinations

human ... RB1(5925)

wet ice

2-8°C

Kaelin, W.G., Functions of the retinoblastoma protein. Bioessays 21, 950-958, (1999)

UN 1789 8/PG 3

Phospho-Retinoblastoma (pThr821) Standard 4 VL

Standard Diluent Buffer 25 mL

Monoclonal Anti-Retinoblastoma (Rb) Antibody-Coated 96 wells plate 1 EA

Anti-Phospho-Retinoblastoma (Rb) (pThr⁸²¹) Antibody 11 mL

Anti-Rabbit IgG-HRP, Concentrate (100X) 1 VL

HRP Diluent 25 mL

Wash Buffer concentrate, 25X 100 mL

Stabilized Chromogen (TMB) 25 mL

Stop Solution 25 mL

Plate Covers, Adhesive Strips 3 EA