Src ELISA human 

The sensitivity is <1 ng/mL, which is as sensitive as immunoblotting. No cross-reactivity is shown with p38, p53, and Akt. The assay has 100% cross-reactivity with Fyn, but not Lyn.

Src ELISA detects and quantifies the level of human Src protein independent of its phosphorylation state from lysates of cells. It can be used to normalize the Src content of the samples when examining quantities of phosphorylated sites on Src using Phospho-Src [pTyr⁴¹⁸] ELISA (CS0460).

Src ELISA is designed to detect and quantify human Src protein. c-Src (pp60c-src), the cellular homolog of the Rous sarcoma virus protein v-Src, is the protein product of the c-src proto-oncogene. Src localizes to the cytoplasm, plasma membrane, focal adhesions, and cadherin-containing cell junctions. Functions include cell adhesion and migration, enhancement of cell survival, and regulation of cell division.

This assay is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates, ready-to-use reagents, and incubation at room temperature.

sufficient for 96 determinations

wet ice

2-8°C

UN 3316 9/PG 3

Src Standard 2 VL

Standard Diluent Buffer 25 mL

Monoclonal-Anti-Src -Coated 96 wells plate 1 EA

Anti-Src Antibody 11 mL

Anti-Rabbit IgG-HRP, Concentrate (100X) 1 VL

HRP Diluent 25 mL

Wash Buffer concentrate, 25X 100 mL

Stabilized TMB Chromogen 25 mL

Stop Solution 25 mL

Plate Covers, Adhesive Strips 3 EA