Analysis Note
The sensitivity is <0.4 ng/mL.
Application
ATF2 ELISA detects and quantifies the level of ATF2 protein. Performance characterization of this ELISA kit was done primarily on human cell lines, cross-reactivity of this kit with mouse cells was observed. ATF2 (activating transcription factor 2) is a member of the ATF/CREB (cAMP response element-binding protein) family of basic region-leucine zipper proteins. Activation of ATF2 is observed in cellular responses to various types of stress and apoptotic signals
ATF2 ELISA is designed to detect and quantify the level of ATF2 protein, independent of its phosphorylation state. It works on human cell lines and cross-reactivity of this kit with mouse cells was observed. This assay is intended for the detection of ATF2 from lysates of cells, and can be used to normalize the ATF2 content of the samples when examining quantities of phosphorylated sites on ATF2 using Sigma Phospho-ATF2 [pThr69/pThr71] ELISA (Product No. CS0550).
General description
ATF2 ELISA is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA) for quantitative determination of ATF2 protein in cell lysates. ATF2 standard dilutions, controls, and unknown samples are added to wells precoated with monoclonal antibody specific for ATF2 (regardless of phosphorylation state) and incubated 2 hours at RT. ATF2 antigen binds to the capture) antibody. Detection Anti- ATF2 antibody is added to the wells and binds to the immobilized ATF2. Anti-IgG- HRP binds to the detection antibody to complete the four-member sandwich. A substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of ATF2 in the original specimen. The optical density measured at 450 nm in the multiwell plate reader is used to calculate the concentration of ATF2
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