Casein Kinase I (CKI) Assay Kit 

Casein Kinase type I (CK-I) is a highly conserved multifunctional monomeric protein kinase of approximately 40 kDa that exists in multiple forms in mammalian tissues. It is present in the nucleus, cytosol, plasma membrane, and microsomes. CK-I is implicated in a variety of cellular functions including vesicular trafficking, DNA repair, cell cycle progression, cytokinesis, glycogen metabolism, and in viral viability. The CK-I family consists of multiple isoforms encoded by seven distinct genes (CK-Iα, β, γ1, γ2, γ3, δ, and ε.). One of these CK-I isoform, CK-Iδ has been implicated in the pathogenesis of Alzeimer's disease.

The Casein Kinase-I Assay Kit provides an easy method for measuring the activity of CK-I by an in vitro phosphorylation of a Casein Kinase I phospho peptide substrate. The sequence of the peptide is based on the glycogen synthase phosphorylation region. The kit contains a CK-I specific inhibitor (IC261), to ensure the specificity of the CK activity measured, and a purified CK-Iδ enzyme, to serve as a positive control. The IC261 inhibitor is most potent against CK-Iδ and CK-Iε and less potent against by CK-Iα. The kit can be used for CK-I activity measurement in cell lysates, tissue homogenates, column fractions, or of purified enzyme.

sufficient for 50 reactions

human ... CSNK1D(1453), CSNK1E(1454), CSNK1G1(53944), CSNK1G2(1455), CSNK1G3(1456)

dry ice

−20°C

Mashhoon, N., et al., Crystal Structure of a Conformation-selective Casein Kinase-1-Inhibitor. J. Biol. Chem. 275, 20052-20060, (2000) abstract

Assay Buffer for Casein Kinase Activity 5 x 1.5 mL

ATP Solution 0.5 mL

IC261 (CKI Inhibitor) 0.5 mL

Casein Kinase 1δ 5 μg

Casein Kinase 1 Phospho Substrate 0.5 μg

Enzyme Dilution Buffer 1.7 mL

P81 Cellulose Phosphate Squares 10 EA