Casein Kinase II Assay Kit 

Casein Kinase type II (CK II) is a tetrameric enzyme of 130-150 kDa with α2β2 structure. The α subunit is catalytic and the β subunit is thought to have regulatory properties. CK II is expressed in the nucleus as well as in the cytoplasm and mitochondria. It has been implicated in a variety of cellular processes and is important for signaling pathways controlling growth division including mitosis, cellular transformation, and differentiation of cells

The Casein Kinase II Assay Kit measures the CKII enzyme activity by in vitro phosphorylation of the Casein Kinase II Substrate. This substrate is a peptide that is selective for CK II with no activity with CK I.
The kit also contains a CK II specific inhibitor to ensure the specificity of the CK activity, and purified CK II for use as a positive control.

sufficient for 70 reactions

human ... CSNK2A1(1457), CSNK2A2(1459), CSNK2B(1460)

dry ice

−20°C

Pinna, L.A., , Casein kinase 2: an 'eminence grise' in cellular regulation? . Biochim. Biophys. Acta 1054, 267-284, (1990) abstract

Assay Buffer for Casein Kinase Activity 1.5 mL

ATP Solution 0.5 mL

Casein Kinase II Substrate 1 mg

Casein Kinase II from Rat Liver 1 UN

Heparin (CKII Inhibitor) 1 mL

Enzyme Dilution Buffer 1.7 mL

P81 Cellulose Phosphate Squares 10 EA