phospho-IκBα (pSer³²) ELISA human 

The sensitivity is <0.5 units/mL, which is 2x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

This assay is designed to detect and quantify the level of IκBα protein phosphorylated on serine 32 in human cells. The kit is not recommended for detection of mouse and rat IκBa. For normalizing the IκBα content of the samples, IκBα ELISA, which is independent of phosphorylation status, is available from Sigma (Product No. CS0620).

IκBα (40 kDa), ubiquitously expressed among mammals, controls immune and inflammatory responses, cell division, and apoptosis. Numerous disease states including arthritis, asthma, and inflammatory bowel disease are associated with loss of IκBα regulation.

The assay is a sensitive and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates and ready-to-use reagents.

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA). The optical density of standards measured at 450 nm in the multiwell plate reader is used to calculate IκBα.concentration in the original specimen.

sufficient for 96 determinations

human ... NFKBIA(4792)

wet ice

2-8°C

UN 3316 9/PG 3

Phospho-IκBα [pSer³²] Standard, Lyophilized 2 VL

Standard Diluent Buffer 25 mL

Monoclonal-Anti- IκBα Coated 96 well plate 1 EA

Anti-Phospho-IκBα [pSer³²], 11 mL

Anti-Rabbit IgG-HRP, Concentrate (100X 1 VL

HRP Diluent 25 mL

Wash Buffer Concentrate, 25X 100 mL

Stabilized TMB Chromogen 25 mL

Stop Solution 25 mL

Plate Covers, Adhesive Strips 3 EA