NDPase (GDPase, UDPase, ADPase) Assay Kit 

The kit was tested on rat tissue extracts (liver, kidney, spleen, and heart) and on a yeast (S. cerevisae) extract. It was also tested with microsomal fractions prepared from rat tissues and cell lines.

A simple and reliable method for measuring NDPase activity in sub-cellular fractions based on the detection of the inorganic phosphate released from NDP by the appropriate NDPase. The color formation, measured at 700 nm in the presence of molybdate, is proportional to the amount of the inorganic phosphate released by enzymes.

1 unit of NDPase releases 1 μmole of inorganic phosphate per minute at 25 °C under conditions specified by the assay.

GDPases/UDPases (NDPases) in yeasts are located exclusively in the Golgi complex and are a convenient marker for Golgi detection. NDPs are degraded to NMPs by nucleoside diphosphatase (GDPase/UDPase). The NMPs are then used in antiport transport of new nucleotide sugars into the organelle lumen. ADPases are known to be expressed on the cell plasma membrane (endothelial cells and vascular precursors) where they participate in nucleotide metabolism.

sufficient for 300 assays (96-well plates)

dry ice

−20°C

Braun, N., et al., Sequencing, functional expression and characterization of rat NTPDase6, a nucleoside diphosphatase and novel member of the ecto-nucleoside triphosphate diphosphohydrolase family. Biochem. J. 351, 639-647, (2000) abstract

UN 3316 9/PG 3

ADPase Assay Reagent 10 mL

GDPase Assay Reagent 10 mL

UDPase Assay Reagent 10 mL

Ascorbic Acid Solution 1 mL

Molybdenum Color Reagent 60 mL

Sodium dodecyl sulfate Solution 10% 5 mL

Phosphorus standard Solution 0.65 mM 1.7 mL