Antioxidant Assay Kit 

The kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.

The antioxidant assay is based on the formation of a ferryl myoglobin radical from myoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation ABTS⁺, a soluble green color chromogen that can be determined at 405 nm. In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox^™, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.

Trolox is a trademark of Hoffman-LaRoche & Co. AG.

sufficient for 200 tests

wet ice

2-8°C

Assay Buffer 10X 30mL

Stop Solution 20mL

Hydrogen Peroxide, 3% solution 1mL

Myoglobin from horse heart 2X1mg

(±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2X1mg

ABTS, 10 mg tablets 4tabs

Phosphate-Citrate Buffer, pH 5 4tabs