Cathepsin D Assay Kit 

The assay is based on the hydrolysis, by the enzyme, of an internally quenched fluorimetric substrate.

Cathepsin D, a ubiquitous aspartic protease belonging to the A1 peptidase family, is found intracellularly in lysosomes. The enzyme has been associated with various biological processes such as: apoptotic events (e.g., the release of cytochrome c from mitochondria and the loss of the transmembrane potential (ΔΨ), aging, Alzheimer's disease, and breast cancer.

• Contains all the reagents required for the measurement of the cathepsin D activity in cell and tissue lysates.
• The bottom limit of detection is 750 pg of protein (or 13 femtomoles of enzyme).
• A single step reaction with direct, real time observation of the degradation product released by the enzyme.
• The kit has been tested on extracts from CHO, HeLa, HEK 293, A431, HepG2, U937, and Jurkat cells on S. cerevisiae and on rat brain, kidney and liver tissues.

sufficient for 120 assays (100 μL)

dry ice

−20°C

UN 3265 8/PG 3

C0715, Monoclonal Anti-Cathepsin D antibody produced in mouse

C3138, Cathepsin D from bovine spleen

C8696, Cathepsin D from human liver

Assay Buffer 1x 20 mL

Cathepsin D from bovine spleen 5 units

Albumin Solution 1 mL

Cathepsin D Substrate Solution 0.25 mL

7-Methoxycoumarin 4-acetic acid Standard Solution 0.1 mL

Pepstatin A Inhibitor Solution 0.6 mL