Apo-TRACE^™ In Vivo Apoptosis Detection Kit 

Apo-TRACE In Vivo Apoptosis Detection Kit is designed for in vivo apoptotic cell staining and quantitative analysis of Apo-TRACE accumulation. Apo-TRACE uptake into apoptotic cells can be evaluated quantitatively by measuring the level of accumulation of the fluorescent dye within the cells of the organ or tissue. The detection of apoptosis by fluorescent methods depends on the labeling efficiency, which varies among cell/tissue types, cell number, or tissue weight, and the sensitivity of the detection instrument used.

Apoptosis, a form of programmed cell death, is a biological process important in normal development as well as pathological states. Apo-TRACE is a small, non-toxic, organic molecule, which is a member of the ApoSense™ family of low molecular weight compounds used for imaging cell death in vivo. The ApoSense compounds respond to alterations in plasma membrane potential and phospholipid scrambling, which are hallmarks of apoptotic cells. Upon systemic administration, the compounds can mark apoptotic cells from the early stages of cell death.^1,2,3 The Apo-TRACE compound has inherent fluorescent properties (excitation at 328 nm and emission at 563 nm) and accumulates in the cytoplasm of apoptotic cells in the living body. Since most anticancer treatments act by inducing apoptosis, staining with Apo-TRACE can give a good indication for the efficiency of the treatment.

Apo-TRACE is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

sufficient for 25 injections

dry ice

−20°C

2. Aloya, R., et al., Molecular imaging of cell death in vivo by a novel small molecule probe. Apoptosis 11, 2089-2101, (2006) abstract

3. Damianovich, M., ApoSense: a novel technology for functional molecular imaging of cell death in models of acute renal tubular necrosis. Eur. J. Nucl. Med. 33, 281-291, (2006)

Reshef, A., et al., Novel molecular imaging of cell death in experimental cerebral stroke. Brain Res. 1144, 156-164, (2007) abstract

CS1110, Apo-TRACE^™, Apoptotic Cell Staining Kit

Apo-TRACE^™ Solution 5 mL

CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 20 mL