Frequently Asked Questions
Application
SIGMA FAST™ DAB (3,3'-Diaminobenzidine tetrahydrochloride) with Metal Enhancer Tablets have been developed for use in immunohistology as a precipitating substrate for the localization of peroxidase activity. The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
General description
SIGMA FAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Reconstitution
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.