Taq DNA Polymerase from Thermus aquaticus 

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl₂ or a 10× reaction buffer without MgCl₂ plus a separate tube of MgCl₂ for titration. The latter option may be necessary to determine optimal conditions for amplification.

Taq DNA Polymerase with 10× reaction buffer containing MgCl₂

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

expressed in Escherichia coli

expressed in Escherichia coli

5 units/μL

suitable for polymerase chain reaction (PCR) and automated sequencing reactions

wet ice

−20°C

, Innis, M.A. et al. PCR Protocols: A Guide to Methods and Applications, (1990)

Innis, M.A. et al. Proc. Natl. Acad. Sci. USA 85, 9436, (1988)

3