RED KlenTaq^® DV ReadyMix^™ 

KlenTaq^® DV ReadyMix^™ is an excellent blend for applications requiring high PCR yields from less complex, thoroughly purified templates.

• Better efficiency and yield than Taq-based formulations, due to the higher processivity of KlenTaq-1
• Magnesium optimization is unnecessary, since KlenTaq works over a broad magnesium range
• Excellent amplification up to 3 kb for less complex templates (e.g., plasmid and lambda DNA) and 1.5 kb on human genomic DNA
• When performing large numbers of PCR reactions, KlenTaq DV ReadyMixes can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent performance
• RED KlenTaq DV ReadyMix provides immediate confirmation that not only has the enzyme been added, but that proper mixing has occurred. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis

KlenTaq^® DV ReadyMixes^™ combine the performance benefits of KlenTaq DNA polymerase with the convenience of a ReadyMix. This ready-to-use mixture of KlenTaq-1 DNA polymerase (a 5′-exo-minus, N-terminal deletion of Taq DNA polymerase), 99% pure deoxynucleotides, reaction buffer and a small amount of a proofreading DNA polymerase is provided in a 2× concentrate. RED KlenTaq DV ReadyMix includes Sigma's proprietary inert red dye.
For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Reaction volumes can be scaled down, if desired.

KlenTaq-1 is more efficient and more processive than either native Taq DNA polymerase or other N-terminal deletions of Taq. This means a higher amount of product is generated with the same number of cycles than when using Taq DNA polymerase.
In RED KlenTaq DV ReadyMix, the inert red dye allows for easy confirmation of enzyme addition and visualization of complete mixing during setup. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. There is no need to add a loading buffer/tracking dye prior to electrophoresis. The red dye migrates at approximately the same rate as a 125 base pair fragment.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

KlenTaq is a registered trademark of Wayne Barnes.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. KlenTaq is a registered trademark of Wayne Barnes. Licensed under U.S. Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

4 units/reaction (50 μl reaction volume)

wet ice

−20°C