D218
Sigma

Monoclonal Anti-Dihydropyridine Receptor (α₁ Subunit) antibody produced in mouse

clone 1A, ascites fluid, buffered aqueous solution

MDL number

MFCD01633297

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Descriptions

Immunogen

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

Specificity

Reacts specifically with the α₁ subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Properties

antibody form

ascites fluid

clone

form

buffered aqueous solution

mol wt

antigen mol wt ~200 kDa

species reactivity

rat, mouse, guinea pig, rabbit, human (weakly)

application(s)

immunoblotting (chemiluminescent): 1:2,000

immunohistochemistry (frozen sections): 1:500

immunoprecipitation: suitable

isotype

IgG1

Gene Information

mouse ... Cacna1s(12292)

rat ... Cacna1s(116652)

human ... CACNA1S(779)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Morton, M.E., et al., Monoclonal antibody identifies a 200-kDa subunit of the dihydropyridine-sensitive calcium channel. J. Biol. Chem. 262, 11904-11907, (1987) abstract

Safety

WGK Germany