D2192
Sigma

Desthiobiotin polyethyleneoxide Iodoacetamide

≥90% (HPLC)

Synonym

Desthiobiotin PEO iodoacetamide

Molecular Formula

C₂₁H₃₈IN₅O₆

Molecular Weight

583.46

MDL number

MFCD06202009

PubChem Substance ID

24893652

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Descriptions

Application

Especially useful for cysteine labeling in many proteomics applications such as peptide mapping and mass spectrometry.

Features and Benefits

• Easily eluted from streptavidin or avidin affinity matrices under mild conditions
• Incorportates a 16 atom hydrophilic spacer
• Typically coupled to sulfhydryl groups at pH 7.5 - 8.5

Other Notes

Sulfhydryl specific, water soluble biotinylation reagent.

Properties

assay

≥90% (HPLC)

form

powder

solubility

H₂O: 10 mg/mL

storage temp.

2-8°C

References

Reference

Conrads, T.P. et al, Quantitative Analysis of Bacterial and Mammalian Proteomes Using a Combination of Cysteine Affinity Tags and ¹⁵N-Metabolic Labeling. Anal. Chem. 73, 2132-2139, (2001) abstract

Goshe, M.B., Blonder, J. and Smith, R.D., Affinity Labeling of Highly Hydrophobic Integral Membrane Proteins for Proteome-Wide Analysis. J. Protein Res 2, 153-161, (2003)

Griffen, T.J. and Aebersold, R., Advances in Proteome Analysis by Mass Spectrometry. J. Biol. Chem. 276, 45497-45500, (2001) abstract

Safety

Hazard Codes

Risk Statements

36/37/38

Safety Statements

26-36/37

WGK Germany