Components
Includes 10× reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
Features and Benefits
• Enhanced amplification on genomic and difficult DNA templates
• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
• Quick recognition and confirmation of appropriate mixing
• No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.
General description
REDTaq Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The dye migrates slightly faster than bromophenol blue at approximately the same rate as a 125 base pair fragment.
The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
Legal Information
REDTaq is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.