D4527
Sigma

Deoxyribonuclease I from bovine pancreas

Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Synonym

Deoxyribonucleate 5′-oligonucleotido-hydrolase

DNase I

CAS Number

9003-98-9

Enzyme Commission (EC) Number

3.1.21.1 ( BRENDA | IUBMB )

MDL number

MFCD00130918

EG/EC Number

232-667-0

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Descriptions

Analysis Note

Protein determined by biuret.

Application

Used for the removal of DNA from protein samples.

Preparation Note

Derived from New Zealand-sourced pancreas

Unit Definition

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg²⁺] = 4.2 mM.

Physical form

Contains calcium chloride

Enhanced Quality Profile

The Enhanced Quality Profile characterizes an individual product's quality traits in a multilevel system to assure that a given product meets the quality requirements of our customers.

Properties

type

Type II

form

lyophilized powder

mol wt

mol wt ~31 kDa

purified by

chromatography

composition

Protein, ≥80%

foreign activity

Chymotrypsin ≤0.01%

Protease ≤0.005%

RNase ≤0.01%

quality classification

Premium

storage temp.

−20°C

References

Reference

Cull, M., and McHenry, C.S., Preparation of extracts from prokaryotes. Meth. Enzymol. 182, 147-153, (1990)

Moore, S. The Enzymes 3rd ed.,, (1981) 14, 281

Molloy, M.P., et al., Proteomic analysis of the Escherichia coli outer membrane. Eur. J. Biochem. 267, 2871-2881, (2000)

Lindberg, U. Biochemistry 6, 335, (1967)

Laskowski, M. The Enzymes 3rd ed.,, (1971) 4, 289

Merck

Merck 13,2923

Safety

Safety Statements

22-24/25

WGK Germany

RTECS

RF0750000

10-21