REDAccuTaq^® LA DNA Polymerase 

REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.

• Increased fidelity, up to 6.5× that of Taq DNA polymerase
• Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA

REDAccuTaq^® LA DNA polymerase is a blend of Sigma's high quality Taq DNA polymerase, a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity, and our inert red dye. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Licensed under U.S. Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.

REDAccuTaq is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

1 unit/μL

wet ice

−20°C

3