D4816
Sigma

DNA Chromatography

1 of 1

General description

This book describes DNA Chromatography a powerful and effective tool for the molecular biologist researcher. The technology is useful for a variety of applications including the analysis of mutations (SNPs, insertions, and deletions), the size determination of PCR products, the purification of single- and double-stranded nucleic acids, analysis of enzymatic reaction products and many others. DNA Chromatography is automated and can replace many of the gel electrophoresis technologies being used resulting in faster and more accurate analysis.

Expand/Collapse All

Identifiers

ISBN-10

3-527-30244-1

ISBN-13

978-3-527-30244-4

Properties

publication info

D.T. Gjerde, et al., Wiley-VCH, 2002, 244 pp., hard cover

Table of Contents

Introduction.
INSTRUMENTATION AND OPERATION.
CHROMATOGRAPHIC PRINCIPLES FOR DNA SEPARATION.
DHPLC.
SIZE BASED SEPARATIONS.
PURIFICATION OF NUCLEIC ACIDS.
RNA CHROMATOGRAPHY.
SPECIAL TECHNIQUES.
LOOKING FORWARD.
Appendix 1: Glossary of Terms.
Appendix 2: System Cleaning and Passivation Treatment.
Appendix 3: Frequently Asked DHPLC Questions.
Index.