D5758
Sigma

Diethyl pyrocarbonate

≥97% (NMR)

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Synonym

DEP

DEPC

Diethyl dicarbonate

Diethyl oxydiformate

Ethoxyformic acid anhydride

Molecular Formula

O(COOC₂H₅)₂

Molecular Weight

162.14

CAS Number

1609-47-8

Beilstein Registry Number

637031

MDL number

MFCD00009106

PubChem Substance ID

24893964

EG/EC Number

216-542-8

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Descriptions

Frequently Asked Questions

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Application

Modification reagent for His and Tyr residues in proteins.

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Properties

assay

≥97% (NMR)

refractive index

n20/D 1.398(lit.)

93-94 °C/18 mmHg(lit.)

density

1.101 g/mL at 25 °C(lit.)

storage temp.

2-8°C

References

Reference

Leonard, N.J., Reaction of diethyl pyrocarbonate with nucleic acid components. I. Adenine. Proc. Natl. Acad. Sci. USA 67, 93, (1970)

, Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Plainview, NY , (1989), 7.3-7.4

Merck

Merck 13,8088

Beilstein

Beil. 3,IV,18

Fieser

Fieser 13,110

reference

Aldrich MSDS 1, 681:A / Corp MSDS 1 (1), 1224:A / FT-IR 2 (1), 977:A / FT-IR 1 (1), 611:B / FT-NMR 1 (1), 929:C / IR-Spectra (3), 365:C / IR-Spectra (2), 325:A / NMR-Reference 2 (1), 517:A / RegBook 1 (1), 695:F / Sax 6, 1390 / Sigma FT-IR 1 (2), 466:C / Structure Index 1, 105:B:6 / Vapor Phase 3, 634:C

Safety

Hazard Codes

Risk Statements

22-36/37/38

Safety Statements

26-36

WGK Germany

RTECS

LQ9350000

Flash Point(F)

156.2 °F

Flash Point(C)