JumpStart^™ AccuTaq^™ LA DNA Polymerase 

JumpStart^® AccuTaq^™ LA DNA Polymerase is a combination of AccuTaq LA DNA Polymerase and a Taq-directed antibody. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot start mechanism provides increased specificity and higher target yield in comparison to standard amplification. JumpStart AccuTaq LA DNA Polymerase can generate long products with higher fidelity (up to 6.5× of Taq DNA polymerase).

• JumpStart Taq DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
• Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.

Supplied with 10× reaction buffer.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

AccuTaq is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Jacobs is a registered trademark of Jacobs.

JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. Licensed under U.S. Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.

2.5 unit/μL

wet ice

−20°C

3