Taq SuperPak^™ DNA Polymerase 

Each set includes:
• Taq DNA polymerase supplied at 5 units/μl
• Optimized 10× Reaction Buffer available with or without MgCl₂. A separate tube of 25 mM MgCl₂ is included with the 10× buffer not containing MgCl₂.
• Ultrapure Deoxynucleotide mix containing 10 mM each of ultrapure dATP, dCTP, dGTP and dTTP in molecular biology grade water. It is suitable for use in routine and specialized (long and accurate) PCR, manual and automated sequencing, RT-PCR and cDNA synthesis reactions, DNA labeling and mutagenesis reactions.

Taq SuperPaks are conveniently packaged to include all reagents needed for PCR except for specific primers, template and water. Also available with REDTaq^® DNA Polymerase.

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

REDTaq is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

SuperPak is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

wet ice

−20°C

3

Taq DNA polymerase (5 units/μl)

10 mM Deoxynucleotide mix

10× PCR buffer without MgCl₂

25 mM MgCl₂