D6064
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DNA Arrays: Methods and Protocols

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General description

Microarray technology is used in research of gene expression, mutation analysis, proteomics and gene sequencing. Protocols provide instruction in designing and constructing DNA arrays as well as hybridizing them with biological samples for analysis. Details include how to attach or print arrays on various matrices, biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies and data collection and analysis. Other topics are genotyping, sequencing by hybridization, antisense reagents, gene expression analysis, and business aspects of biochip technology.

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Identifiers

ISBN-10

0-89603-822-X

ISBN-13

978-0-89603-822-6

Properties

publication info

J.B. Rampal, ed., Humana Press, 2001, 264 pp., hard cover

Table of Contents

DNA Microarrays: History and Overview
Gel-Immobilized Microarrays of Nucleic Acids and Proteins: Production and Application for Macromolecular Research
Sequencing by Hybridization Arrays
Ethical Ramifications of Genetic Analysis Using DNA Arrays
Photolithographic Synthesis of High-Density Oligonucleotide Arrays
Automated Genotyping Using the DNA MassArrayTM Technolog
Ink-Jet–Deposited Microspot Arrays of DNA and Other Bioactive Molecules
Printing DNA Microarrays Using the Biomek 2000 Laboratory Automation Workstation
Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays
Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays
DNA Sequencing by Hybridization with Arrays of Samples or Probes,
Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents
Low-Resolution Typing of HLA-DQA1 Using DNA Microarray
Gene Expression Analysis on Medium-Density Oligonucleotide Arrays
Use of Bioinformatics in Arrays
Confocal Scanning of Genetic Microarrays
Business Aspects of Biochip Technologies.
Index.