Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse 

outer arm dynein intermediate chain of Chlamydomonas.

Recognizes the intermediate chain (IC69, 69 kDa) of Chlamydomonas dynein, but does not recognize any other protein in the axoneme. Reacts strongly with the 76 kDa intermediate chain outer arm dynein of sea urchin (Tripneustes gratilla, Strongylocentrotus purpuratus) and with a single component (IC2, 73 kDa) of trout (Salmo gairdneri) outer arm dynein. Does not recognize any component in axonemes prepared from scallop gill cilia or sheep sperm, purified 14S and 22S dyneins from cilia of Tetrahymena thermophila, or chick brain extract.

May be used for domain mapping within IC69, electron microscope studies on dynein particles, and for protein-protein association studies on purified outer arm dynein and axonemes from Chlamydomonas flagella.

ascites fluid

1869A

15 mM sodium azide

Chlamydomonas, sea urchin (Tripneustes gratilla), Strongylocentrotus purpuratus, trout (Salmo gairdneri)

immunoblotting: 1:10,000 using Chlamydomonas flagella

immunoblotting: 1:10000 using Chlamydomonas reinhardtii flagella preparation

IgG1

dry ice

−20°C

King, S.M., and Witman, G.B., Warner, P., et al., ed. Cell Movement: The Dynein ATPases New York , (1989) 1, 61

King, S.M. J. Biol. Chem. 266, 8401, (1991) abstract

King, S.M. Cell Motil. Cytoskeleton 16, 266, (1990) abstract

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