DOTAP chloride 

Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.

≥99% (TLC)

lyophilized powder

dry ice

−20°C

Lu, D., et al., Optimization of methods to achieve mRNA-mediated transfection of tumor cells in vitro and in vivo employing cationic liposome vectors. Cancer Gene Ther. 1, 245-252, (1994)

Simoes, S., et al., Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusigenic peptides. Gene Ther. 5, 955-964, (1998)

Anchordoquy, T.J., et al., Stability of lipid/DNA complexes during agitation and freeze-thawing. J. Pharm. Sci. 87, 1046-1051, (1998)

Bei, R., et al., The use of a cationic liposome formulation (DOTAP) mixed with a recombinant tumor-associated antigen to induce immune responses and protective immunity in mice. J. Immunother. 21, 159-169, (1998)

Prasmickaite, L., et al., Intracellular metabolism of a 2'-O-methyl-stabilized ribozyme after uptake by DOTAP transfection or as free ribozyme. A study by capillary electrophoresis. Nucleic Acids Res. 26, 4241-4248, (1998)

Birchall, J.C., et al., Physico-chemical characterisation and transfection efficiency of lipid-based gene delivery complexes. Int. J. Pharmacol. 183, 195-207, (1999)

Crook, K., et al., Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum. Gene Ther. 5, 137-143, (1998)

Goldberg, G.S. and Lau, A.F., Transfection of mammalian cells with PEG-purified plasmid DNA. Biotechniques 14, 548-550, (1993)

Yang, J.P., and Huang., L., Time-dependent maturation of cationic liposome-DNA complex for serum resistance. Gene Ther. 5, 380-387, (1998)

Li, S., et al., Characterization of cationic lipid-protamine-DNA (LPD) complexes for intravenous gene delivery. Gene Ther. 5, 930-937, (1998)

Zelphati, O., Szoka, F.C., Jr., Intracellular distribution and mechanism of delivery of oligonucleotides mediated by cationic lipids. Pharmacol. Res. 13, 1367-1372, (1996)

Holmen, S.L., et al., Efficient lipid-mediated transfection of DNA into primary rat hepatocytes. In Vitro. Cell Dev. Biol. Anim. 31, 347-351, (1995)

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