JumpStart^™ Taq ReadyMix^™ for High Throughput Quantitative PCR 

• To prepare a typical QPCR reaction, mix 25 μl of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μl. Reaction volumes can be scaled down, if desired.
• JumpStart Taq DNA polymerase prevents non-specific product formation, and allows assembled PCR reactions to be placed at room temperature for up to two hours without compromising the performance.
• Internal Reference Dye is provided for reaction normalization.
• When performing large numbers of PCR reactions, JumpStart ReadyMix Taq can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent batch-to-batch and reaction-to-reaction performance.

JumpStart Taq ReadyMix For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. The ReadyMix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. It is provided in a 2× concentrate. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

2.5 units/rxn

wet ice

−20°C

Z702439, A-Z of Quantitative PCR