JumpStart^™ Taq ReadyMix^™ for Quantitative PCR 

• Ultimate Convenience. ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template
• Greater Specificity & Increased Target Yield. JumpStart Taq prevents non-specific amplification and increased target yield
• Maximum Flexibility. Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR^® Green I

JumpStart Taq ReadyMix for Quantitative PCR combines the advantages of a hot start enzyme with a ready-to-use mix for high throughput, quantitative PCR (QPCR). It is formulated without a detection chemistry making it suitable for use with a variety of formats including dual-labeled probes, molecular beacons, or double stranded binding dyes such as SYBR^® Green I.

Sigma's Reference Dye for Quantitative PCR is included separately with this ReadyMix for normalization of the reaction data. The dye has a maximum excitation of 586 nm, and a maximum emission of 605 nm. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. A tube of 25 mM MgCl₂ is also provided for easy optimization of the qPCR reaction.

The ReadyMix contains JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, and buffer in an optimized 2× concentrate. To prepare a reaction, 25 μL of the ReadyMix is added to primers, template, detection chemistry, and water for a total reaction volume of 50 μL. Set up can be performed at room temperature since the JumpStart Taq antibody renders the Taq DNA polymerase inactive. During the first denaturation cycle, the antibody dissociates from the enzyme and full activity is restored. No special preparations or protocol changes are required.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

SYBR is a registered trademark of Molecular Probes, Inc.

1.5 units/reaction (50 μL reaction volume)

wet ice

−20°C

3

Z702439, A-Z of Quantitative PCR

JumpStart^™ Taq ReadyMix^™ P2893

25 mM MgCl₂

Reference Dye for Quantitative PCR, 100 ×, solution R4526