MTP^™ Taq DNA Polymerase 

MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has 5′-3′ DNA polymerase and exonuclease activities, is approximately 95 kD by SDS-PAGE, and has no detectable endonuclease or 3′-5′ exonuclease activities. Contaminating DNA present in most other polymerase preparations often precludes or obscures the accurate interpretation of results, especially when targeting conserved sequences (e.g. bacterial 16S rRNA region)¹.

While MTP Taq is a high-quality, low-contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents². To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq buffer (Sigma product M 9943) with each tube of MTP Taq DNA Polymerase. Each lot of MTP Taq and 10× MTP Taq buffer undergoes the same strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA Polymerase.

View more detailed information on MTP Taq DNA Polymerase at www.sigma-aldrich.com/mtptaq.

Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.

MTP is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

wet ice

−20°C

1. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Kaczmarski EB, Fox AJ, Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. . J. Clin. Microbiol. 38, 1747-1752, (2000) abstract

2. Millar, B.C., Xu, J., and Moore, J.E. , Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. . J. Clin. Microbiol. 40, 1575-1580