Dextran sulfate sodium salt from Leuconostoc spp. 

Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Tested for suitability in nucleic acid hybridizations

for molecular biology

powder

average M_w >500,000 (dextran starting material)

0.5-2% phosphate buffer

H₂O: 100 mg/mL

2-8°C

Wahl, G.M., et al., Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc. Natl. Acad. Sci. USA 76, 3683, (1979) abstract

Lederman, L.L., et al., The rate of nucleic acid annealing to cytological preparations is increased in the presence of dextran sulfate. Anal. Biochem. 117(1), 158-163, (1981)

Merck 13,2969

2

HH9290000