D9170
Sigma

Dimethyl sulfoxide

PCR Reagent

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Synonym

DMSO

Methyl sulfoxide

Molecular Formula

(CH₃)₂SO

Molecular Weight

78.13

CAS Number

67-68-5

Beilstein Registry Number

506008

MDL number

MFCD00002089

PubChem Substance ID

24894271

EG/EC Number

200-664-3

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Descriptions

Application

Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Properties

grade

PCR Reagent

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

autoignition temp.

573 °F

expl. lim.

42 %± , 63 °F

packaging

vial of 1 mL

refractive index

n20/D 1.479(lit.)

189 °C(lit.)

16-19 °C(lit.)

density

1.10 g/mL(lit.)

References

Reference

Cheng, S., et al., Effective amplification of long targets from cloned inserts and human genomic DNA. Proc. Natl. Acad. Sci. USA 91, 5695-5699, (1994) abstract

Winship, P.R., et al., An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Res. 17, 1266, (1989) abstract

Merck

Merck 13,3285

Beilstein

Beil. 1,IV,1277

Fieser

Fieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149

reference

Aldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A

Safety

WGK Germany

RTECS

PV6210000

UEL

28.50%(V)

LEL

2.60%(V)

Flash Point(F)

203 °F

Flash Point(C)

95 °C