JumpStart^™ Taq DNA Polymerase 

• Greater Specificity & Increased Target Yield. JumpStart Taq ReadyMixes, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• Enhanced Sensitivity. JumpStart Taq DNA Polymerases provide superior amplification regardless of template concentration
• Ultimate Convenience. Reduce set-up time and eliminate concerns associated with manual or wax Hot Start methods

Sigma's JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Supplied with 10× reaction buffer containing 15 mM MgCl₂

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl_2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl₂ for optimization.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

2.5 units/μL

wet ice

−20°C