E2760
Sigma

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone 29.1, ascites fluid

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MDL number

MFCD00162282

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Descriptions

Immunogen

human carcinoma cell line A431, which expresses high levels of EGF receptors.

Specificity

Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.

Application

May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis.

Properties

antibody form

ascites fluid

clone

29.1

contains

15 mM sodium azide

species reactivity

human, pig, canine, mouse, rat

does not react with

rabbit

application(s)

immunoblotting: 1:3,000 using extract of the A431 cell line

indirect ELISA: 1:10,000 using extract of the A431 cell line

isotype

IgG1

Gene Information

mouse ... Egfr(13649)

human ... EGFR(1956)

rat ... Egfr(24329)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Schlessinger, J., Greaves, M.F. Monoclonal Antibodies to Receptors: Probes for Receptor Structure and Function, (Receptors and Recognition, Series B, Vol. 17), (1984), 280

Gooi, H.C., et al., The carbohydrate specificities of the monoclonal antibodies 29.1, 455 and 3C1B12 to the epidermal growth factor receptor of A431 cells. Biosci. Rep. 5, 83-94, (1985) abstract

Yarden, Y., et al., Purification of an active EGF receptor kinase with monoclonal antireceptor antibodies. J. Biol. Chem. 260, 315, (1985) abstract

Safety

WGK Germany

Related Categories

... Cytokines & Growth Factors > Antibodies to Epidermal Growth Factors

... Antibodies to Cytokines and Growth Factors > Antibodies to Epidermal Growth Factors (EGF) and NRG Family

... Alphabetical Index > E

... Epidermal Growth Factors (EGF) and NRG Family > EGF Receptors

... Receptor Tyrosine Kinase Antibodies > Epidermal Growth Factor Receptor (EGFR)

... Tumor Growth Regulation > Epidermal Growth Factor Receptors

... Clone Index > 2

E2760 - Datasheet (30kb)

MSDS

Certificate of Analysis

More Information