Anti-EB1 antibody produced in rabbit 

synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

affinity isolated antibody

buffered aqueous solution

antigen mol wt 35 kDa

human, rat, mouse, canine, bovine, pig

~1.5 mg/mL

immunoblotting: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1

immunoprecipitation: 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line

indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells

dry ice

−20°C

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